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m 280 streptavidin magnetic beads  (Thermo Fisher)


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    Structured Review

    Thermo Fisher m 280 streptavidin magnetic beads
    M 280 Streptavidin Magnetic Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m+280+streptavidin+magnetic+beads/Streptavidin/pmc13019968-302-23-27
    Average 99 stars, based on 1 article reviews
    m 280 streptavidin magnetic beads - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Acyl Carrier Protein is Essential for Apicoplast Biogenesis in Malaria Parasites Independent of Fatty Acid Synthesis
    Article Snippet: .. Parasite pellets were lysed in 1 mL 1% Triton X-100/PBS and clarified by centrifugation at 17,000 x g. Clarified lysates were added to 25 μL of M-280 streptavidin magnetic beads (Invitrogen 11205D) that had been equilibrated in PBS, incubated overnight with rotating at 4° C, and washed 5x with 1 mL 1% Triton/PBS, 4x with 1 mL 8M urea/PBS, and 2x in PBS ensuring that samples were moved to new tubes between each wash condition. ..

    Magnetic Beads:

    Article Title: Acyl Carrier Protein is Essential for Apicoplast Biogenesis in Malaria Parasites Independent of Fatty Acid Synthesis
    Article Snippet: .. Parasite pellets were lysed in 1 mL 1% Triton X-100/PBS and clarified by centrifugation at 17,000 x g. Clarified lysates were added to 25 μL of M-280 streptavidin magnetic beads (Invitrogen 11205D) that had been equilibrated in PBS, incubated overnight with rotating at 4° C, and washed 5x with 1 mL 1% Triton/PBS, 4x with 1 mL 8M urea/PBS, and 2x in PBS ensuring that samples were moved to new tubes between each wash condition. ..

    Article Title: A conserved mammalian mecciRNA, mecciATP6, regulates mitochondrial homeostasis through interaction with HNRNPA3
    Article Snippet: .. The supernatant was collected, and 100 pmol of biotinylated antisense oligonucleotide was added, followed by incubation at room temperature for 2 h. Concurrently, M-280 streptavidin magnetic beads (Life Technologies) were blocked by incubating with 500 ng/μL yeast tRNA and 500 ng/μL BSA at room temperature for 1 h, washed with RIPA buffer, and resuspended. .. For every 100 pmol of biotin-DNA oligonucleotide, 50 μL of the blocked beads were added to the mixture, which was then incubated with rotation at room temperature for 2 h. The beads were collected using a magnetic stand (Life Technologies) and washed twice with RIPA buffer, followed by two washes with RIPA buffer supplemented with 500 mmol/L NaCl.

    Article Title: Long non‑coding RNA ABHD11‑AS1 inhibits colorectal cancer progression through interacting with EGFR to suppress the EGFR/ERK signaling pathway.
    Article Snippet: Long non‐coding (lnc)RNAs participate in colorectal cancer (CRC) occurrence and progression.. The present study aimed to investigate whether lncRNA ABHD11‐AS1 regulates malignant biological behavior of CRC cells.. Bioinformatic analysis, reverse transcription‐quantitative PCR and in situ hybridization revealed that ABHD11‐AS1 expression was decreased in CRC samples and associated with an unfavor‐ able prognosis.

    Article Title: IL-6-induced long noncoding RNA MIR3142HG promotes tumorigenesis by interacting with thioredoxin-1 and STAT3 in human colorectal cancer.
    Article Snippet: .. M-280 Streptavidin magnetic beads (Invitrogen) were added to the cell lysate and then incubated for 1 h at room temperature (RT) with rotation. ..

    Article Title: IL-6-induced long noncoding RNA MIR3142HG promotes tumorigenesis by interacting with thioredoxin-1 and STAT3 in human colorectal cancer
    Article Snippet: .. M-280 Streptavidin magnetic beads (Invitrogen) were added to the cell lysate and then incubated for 1 h at room temperature (RT) with rotation. ..

    Article Title: Long non-coding RNA ABHD11-AS1 inhibits colorectal cancer progression through interacting with EGFR to suppress the EGFR/ERK signaling pathway
    Article Snippet: .. ABHD11-AS1 intercalating proteins were captured using 40 μ l M-280 streptavidin magnetic beads (cat. no. 11205D, Invitrogen; Thermo Fisher Scientific, Inc.) and target proteins were subsequently identified by western blotting (anti-EGFR, 1:1,000, A11577, ABclonal; anti-GAPDH, 1:1,000, 10494-1-AP, Proteintech Group, Inc.). ..

    Article Title: LncRNA AK020546 Alleviates Myocardial Ischemia-Reperfusion Injury via Suppressing METTL14/m6A/MST1 Axis-Mediated Ferroptosis.
    Article Snippet: Ferroptosis occurs after myocardial ischemia–reperfusion (I/R) injury.. Long non-coding RNA (lncRNA) AK020546 possesses the effect of protecting the myocardium from I/R injury; however, the underlying mechanisms remain not fully understood.. This study aimed to investigate the effect of AK020546 on ferroptosis in cardiomyocytes and underlying molecular mechanisms.

    Article Title: SNHG19 promotes the proliferation and metastasis of hepatocellular carcinoma through regulating the miR-137/protein tyrosine phosphatase 4A3 axis
    Article Snippet: .. After transfection, cells were cultured with M-280 streptavidin magnetic beads (Invitrogen, USA). ..

    Incubation:

    Article Title: Acyl Carrier Protein is Essential for Apicoplast Biogenesis in Malaria Parasites Independent of Fatty Acid Synthesis
    Article Snippet: .. Parasite pellets were lysed in 1 mL 1% Triton X-100/PBS and clarified by centrifugation at 17,000 x g. Clarified lysates were added to 25 μL of M-280 streptavidin magnetic beads (Invitrogen 11205D) that had been equilibrated in PBS, incubated overnight with rotating at 4° C, and washed 5x with 1 mL 1% Triton/PBS, 4x with 1 mL 8M urea/PBS, and 2x in PBS ensuring that samples were moved to new tubes between each wash condition. ..

    Article Title: A conserved mammalian mecciRNA, mecciATP6, regulates mitochondrial homeostasis through interaction with HNRNPA3
    Article Snippet: .. The supernatant was collected, and 100 pmol of biotinylated antisense oligonucleotide was added, followed by incubation at room temperature for 2 h. Concurrently, M-280 streptavidin magnetic beads (Life Technologies) were blocked by incubating with 500 ng/μL yeast tRNA and 500 ng/μL BSA at room temperature for 1 h, washed with RIPA buffer, and resuspended. .. For every 100 pmol of biotin-DNA oligonucleotide, 50 μL of the blocked beads were added to the mixture, which was then incubated with rotation at room temperature for 2 h. The beads were collected using a magnetic stand (Life Technologies) and washed twice with RIPA buffer, followed by two washes with RIPA buffer supplemented with 500 mmol/L NaCl.

    Article Title: IL-6-induced long noncoding RNA MIR3142HG promotes tumorigenesis by interacting with thioredoxin-1 and STAT3 in human colorectal cancer.
    Article Snippet: .. M-280 Streptavidin magnetic beads (Invitrogen) were added to the cell lysate and then incubated for 1 h at room temperature (RT) with rotation. ..

    Article Title: IL-6-induced long noncoding RNA MIR3142HG promotes tumorigenesis by interacting with thioredoxin-1 and STAT3 in human colorectal cancer
    Article Snippet: .. M-280 Streptavidin magnetic beads (Invitrogen) were added to the cell lysate and then incubated for 1 h at room temperature (RT) with rotation. ..

    Article Title: LncRNA AK020546 Alleviates Myocardial Ischemia-Reperfusion Injury via Suppressing METTL14/m6A/MST1 Axis-Mediated Ferroptosis.
    Article Snippet: Ferroptosis occurs after myocardial ischemia–reperfusion (I/R) injury.. Long non-coding RNA (lncRNA) AK020546 possesses the effect of protecting the myocardium from I/R injury; however, the underlying mechanisms remain not fully understood.. This study aimed to investigate the effect of AK020546 on ferroptosis in cardiomyocytes and underlying molecular mechanisms.

    Western Blot:

    Article Title: Long non‑coding RNA ABHD11‑AS1 inhibits colorectal cancer progression through interacting with EGFR to suppress the EGFR/ERK signaling pathway.
    Article Snippet: Long non‐coding (lnc)RNAs participate in colorectal cancer (CRC) occurrence and progression.. The present study aimed to investigate whether lncRNA ABHD11‐AS1 regulates malignant biological behavior of CRC cells.. Bioinformatic analysis, reverse transcription‐quantitative PCR and in situ hybridization revealed that ABHD11‐AS1 expression was decreased in CRC samples and associated with an unfavor‐ able prognosis.

    Article Title: Long non-coding RNA ABHD11-AS1 inhibits colorectal cancer progression through interacting with EGFR to suppress the EGFR/ERK signaling pathway
    Article Snippet: .. ABHD11-AS1 intercalating proteins were captured using 40 μ l M-280 streptavidin magnetic beads (cat. no. 11205D, Invitrogen; Thermo Fisher Scientific, Inc.) and target proteins were subsequently identified by western blotting (anti-EGFR, 1:1,000, A11577, ABclonal; anti-GAPDH, 1:1,000, 10494-1-AP, Proteintech Group, Inc.). ..

    Real-time Polymerase Chain Reaction:

    Article Title: LncRNA AK020546 Alleviates Myocardial Ischemia-Reperfusion Injury via Suppressing METTL14/m6A/MST1 Axis-Mediated Ferroptosis.
    Article Snippet: Ferroptosis occurs after myocardial ischemia–reperfusion (I/R) injury.. Long non-coding RNA (lncRNA) AK020546 possesses the effect of protecting the myocardium from I/R injury; however, the underlying mechanisms remain not fully understood.. This study aimed to investigate the effect of AK020546 on ferroptosis in cardiomyocytes and underlying molecular mechanisms.

    Transfection:

    Article Title: SNHG19 promotes the proliferation and metastasis of hepatocellular carcinoma through regulating the miR-137/protein tyrosine phosphatase 4A3 axis
    Article Snippet: .. After transfection, cells were cultured with M-280 streptavidin magnetic beads (Invitrogen, USA). ..

    Cell Culture:

    Article Title: SNHG19 promotes the proliferation and metastasis of hepatocellular carcinoma through regulating the miR-137/protein tyrosine phosphatase 4A3 axis
    Article Snippet: .. After transfection, cells were cultured with M-280 streptavidin magnetic beads (Invitrogen, USA). ..



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